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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 1, 2016, pp. 19-26
Bioline Code: pr16003
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 1, 2016, pp. 19-26

 en Ulmus davidiana check for this species in other resources var japonica Attenuates Neuroinflammatory Responses in Activated BV2 Murine Microglial Cells via Nuclear Factor-KappaB Signaling Pathway
Chang, Hyun-Joo & Kang, Hyun


Purpose: To investigate the antioxidant and anti-inflammatory effects of Ulmus davidiana check for this species in other resources extract (UDE) in lipopolysaccharide (LPS)-stimulated BV-2 cells.
Methods: Antioxidant activity was measured using 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay. Cell viability was evaluated using 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide (MTT) assay. BV-2 cells were stimulated with LPS to study protein expression and production of inflammatory mediators, and determined by Western blot analysis.
Results: UDE significantly inhibited DPPH-generated free radicals showing maximum inhibition at 40 μg/mL (p < 0.001). UDE alone did not exhibit any signs of cytotoxicity towards BV-2 cells up to 100 μg/mL concentration. The LPS-induced increase in the production of nitric oxide was concentrationdependently suppressed with half-maximal concentration (IC50) of 67.4 μg/ mL of UDE (p < 0.05 at 10 μg/mL, p < 0.01 at 20 μg/mL and p < 0.001 at 40 μg/mL, respectively). UDE also inhibited dosedependently the LPS-induced increase in inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions with IC50 of 52.3 ug/ mL. Furthermore, the production of pro-inflammatory cytokines, via tumor necrosis factor-α by LPS-stimulation in BV2 murine cells was inhibited dosedependently with IC50 of 85.1 ug/ mL by UDE pretreatment. Mechanistic studies revealed that UDE acts by regulation of nuclear factor kappa-B signaling pathway in LPS-stimulated BV-2 cells.
Conclusion: This study shows, for the first time, that UDE possesses antioxidant and anti-inflammatory effects and can be developed as a potential therapeutic agent for ameliorating macrophage-mediated inflammation.

Ulmus davidiana; Anti-oxidant; Anti-inflammatory; iNOS; NF-κB; MCP-1; ERK

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