Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 15, No. 1, 2016, pp. 47-53
Bioline Code: pr16007
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 15, No. 1, 2016, pp. 47-53
© Copyright 2016 - Tropical Journal of Pharmaceutical Research
Aldehyde Dehydrogenase 1 and Raf Kinase Inhibitor Protein Expression Defines the Proliferative Nature of Cervical Cancer Stem Cells|
Sun, Xiang-Xiu; Ma, Qing-Shan; Liu, Tian-Feng; Chen, Ying; Dong, Yan & Zhang, Lin-Lin
Purpose: To determine the differences in response following the use of aldehyde dehydrogenase 1
(ALDH1) and Raf kinase inhibitor protein (RKIP) as cervical cancer stem cell markers.
Methods: To evaluate the cancer stem cell markers, a mouse model with low and high grade cervical
cancer was developed and studied by histological examination. Immunohistochemical and Western
blotting techniques were employed to study the expression profiles of ALDH1 and RKIP. The specificity
of Sox2 that determines cancer stem cells served as control to validate ALDH1 and RKIP expressions.
Results: Histological data helped to differentiate low from high grade cervical cancer. The results from
immunohistochemistry show increased pattern of Sox2 expression as tumour progresses. Similarly,
ALDH1, a protein that positively regulates stem cells shows mild expression in low grade cervical
tumour, but positive signals are more amplified in an aggressive stage of tumour condition when
compared with Sox2. The expression study with RKIP, a protein that negatively regulates stem cells,
interestingly defines the higher expression in low grade cervical cancer to regulate the tumour, but
shows little or no very mild expression in the aggressive stage of tumour. All the data obtained show a
statistically significant value of p < 0.05. The results with Immunohistochemistry were further validated
using the western blotting analysis and it also confirms the similar results.
Conclusion: ALDH1 and RKIP marker in association correlation with Sox2 aids in defining the
proliferative ability of cervical cancer stem cells and also to validate them in initial and advanced stages
of cervical cancer.
Cervical cancer; ALDH1; BALB/c-nu/nu; HeLa cells; RKIP; Sox2
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