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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 1, 2016, pp. 87-94
Bioline Code: pr16012
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 1, 2016, pp. 87-94

 en Antidiabetic Activity of Plumeria Alba check for this species in other resources Linn (Apocynaceae) Root Extract and Fractions in Streptozotocin-Induced Diabetic Rats
Kadébé, Z. Tessou; Metowogo, Kossi; Bakoma, Batomayena; Lawson-Evi, S. Poevi; Eklu-Gadegbeku, Kwashie; Aklikokou, Kodjo & Gbeassor, Messanvi

Abstract

Purpose: To investigate the effect of total hydroalcohol root extract of Plumeria alba check for this species in other resources (Apocynaceae) and its active fraction on diabetes in rats.
Methods: The effect of total hydroalcohol extract at 250 mg/kg and different fractions was evaluated on oral glucose tolerance test (OGTT) in mice. The effect of the active fractions on OGTT was measured on a model of diabetic rats (fructose-enriched fat diet and streptozotocin-induced).
Results: The results show that the total extract (250 mg/kg), ethyl acetate fraction and supernatant fraction (obtained after centrifugation of total extract) at a dose of 100 mg/kg significantly (p < 0.01) reduced hyperglycemia induced by glucose overload in mice. Fructose-enriched fat diet increased blood cholesterol, triglycerides and high density lipoprotein (HDL) levels in hyperlipidemic untreated rats compared to normal control rats. Administration of total extract (250 mg/kg/day) and supernatant fraction of P. alba (100 mg/kg/day) during 14 days significantly reduced lipid parameters (total cholesterol, p < 0.001; triglycerides, p < 0.01; HDL, p < 0.05). Analysis of oxidative stress markers shows that the supernatant fraction and total extract significantly increased serum glutathione level (p < 0.01) but significantly lowered malondialdehyde (MDA) concentration in liver (p < 0.05).
Conclusion: These results suggest that the total extract and fractions of P. alba exhibit significant antidiabetic and hypolipidemic properties in streptozotocin-induced diabetic animals. The supernatant fraction (which is free of organic solvent) was the most biologically active

Keywords
Diabetes; Hyperlipidermia; Plumeria alba; Fructose-enriched fat diet; Oxidative stress markers; Streptozotocin

 
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