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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996 EISSN: 1596-5996
Vol. 15, No. 1, 2016, pp. 95-99
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Bioline Code: pr16013
Full paper language: English
Document type: Research Article
Document available free of charge
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Tropical Journal of Pharmaceutical Research, Vol. 15, No. 1, 2016, pp. 95-99
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Wortmannin as Targeted Therapeutic Agent for the Treatment of Triple-Negative Breast Cancer
Li, Jian; Liu, Fei; Liu, Yang; Du, Jia-Jun & Liu, Qi
Abstract
Purpose: To investigate the inhibitory effect of wortmannin on inhibition of BT-20 and BT-474 breast
cancer cells in Athymic nu/nu mice model.
Methods: Forty Athymic nu/nu mice were randomly assigned to 4 groups of 10 each, namely, BT-20
control, BT-20 treatment, BT-474 control and BT-474 treatment groups. The mice were injected with 2.5
x 105 BT-20 and BT-474 cells under anesthesia. Those in the treatment groups were given wortmannin
(7 mg/kg) in DMSO daily intraperitoneally whereas the animals in control groups received an equal
volume of DMSO for 21 days after the cancer attained palpable stage. Western blot analysis was
carried out using enhanced chemiluminescence reagent while Protean IEF cell unit was used for 1-D
electrophoresis.
Results: The results showed a significant decrease in the growth of the tested cancer cell lines on
treatment with wortmannin at 7 mg/kg daily for 21 days. The volume of tumor in the treatment group
was reduced to 42.72 ± 9.45 compared to 79.43 ± 17.11 mm3 in the control group after 21 days
treatment (p < 0.005). It also changed the expression of microRNA in BT-20 cells. Exposure of BT-20
cells to wortmannin for 24 h resulted in the altered expression of proteomes. Wortmannin treatment
increased the expression of miRs including miR 19a/b, 22, 29b/c, 181c/d, 195 and 663. Western blot
data showed increase in the expression of NME1 (NM23 H1) and reduction in the expression of
vimentin after treatment with wortmannin.
Conclusion: Wortmannin can be of benefit in the treatment of human breast cancer.
Keywords
Wortmannin; Vimentin; Tumor growth; Expression of microRNA; Proteomes
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