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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 1, 2016, pp. 133-140
Bioline Code: pr16019
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 1, 2016, pp. 133-140

 en Evaluation of Antioxidant and Antifungal Activities of Polyphenol-rich Extracts of Dried Pulp of Garcinia pedunculata check for this species in other resources Roxb. and Garcinia morella check for this species in other resources Gaertn. (Clusiaceae)
Sarma, Rahul; Das, Minakshi; Mudoi, Tiluttama; Sharma, Kaustav Kalyan; Kotoky, Jibon & Devi, Rajlakshmi

Abstract

Purpose: To evaluate the antioxidant and antifungal activities of polyphenol-rich extracts of the dried fruit pulp of Garcinia pedunculata check for this species in other resources (GP) and Garcinia morella check for this species in other resources (GM) to determine their traditional claims of therapeutic activity against certain diseases.
Methods: Analysis of total phenolic (TP) and flavonoid (TF) contents of the extracts were performed by Folin-Ciocalteau and Arvouet-Grand methods. The antioxidant activity of the extracts was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2) free radical scavenging activity, reducing power and in vitro lipid peroxidation (LPO). Antifungal activity was evaluated by agar-well diffusion method while mineral content was evaluated by atomic absorption spectrophotometry (AAS).
Results: Significant amounts of TP (5.87 ± 0.06 and 5.46 ± 0.02 mg catechin eqivalents/g) and TF (5.61 ± 0.16 and 3.69 ± 0.04 mg quercetin equivalents/g) were found in the cold water (CW) extracts of GP and GM, respectively, along with DPPH free radical scavenging activity (50 % inhibitory concentration (IC50) = 3.53 ± 0.04 and 1 ± 0.03μg/mL) and H2O2-radical scavenging activity (IC50 = 1.4 ± 0.02 and 1.44 ± 0.01 μg/mL). Results indicated that the CW extracts of GP and GM were potent reducing agent than the HW extracts. CW extract of both species prevented in vitro LPO (IC50= 42 ± 0.01 and 30.36 ± 0.03 μg/mL) significantly. The antifungal activity of GP and GM extracts against some human dermatophytes was high. High concentrations of K and Fe were found in the extracts.
Conclusion: GP and GM extracts have great potential as a source for useful antioxidant and antifungal agents.

Keywords
Antioxidant; Phenolic; Flavonoid; Lipid peroxidation; Antifungal; Dermatophytes

 
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