To evaluate the antioxidant and antifungal activities of polyphenol-rich extracts of the dried
fruit pulp of Garcinia pedunculata
(GP) and Garcinia morella
(GM) to determine their traditional claims of
therapeutic activity against certain diseases.
Analysis of total phenolic (TP) and flavonoid (TF) contents of the extracts were performed by
Folin-Ciocalteau and Arvouet-Grand methods. The antioxidant activity of the extracts was determined
by 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide (H2
) free radical scavenging activity,
reducing power and in vitro lipid peroxidation (LPO). Antifungal activity was evaluated by agar-well
diffusion method while mineral content was evaluated by atomic absorption spectrophotometry (AAS).
Significant amounts of TP (5.87 ± 0.06 and 5.46 ± 0.02 mg catechin eqivalents/g) and TF
(5.61 ± 0.16 and 3.69 ± 0.04 mg quercetin equivalents/g) were found in the cold water (CW) extracts of
GP and GM, respectively, along with DPPH free radical scavenging activity (50 % inhibitory
) = 3.53 ± 0.04 and 1 ± 0.03μg/mL) and H2
-radical scavenging activity (IC50
= 1.4 ±
0.02 and 1.44 ± 0.01 μg/mL). Results indicated that the CW extracts of GP and GM were potent
reducing agent than the HW extracts. CW extract of both species prevented in vitro LPO (IC50
= 42 ±
0.01 and 30.36 ± 0.03 μg/mL) significantly. The antifungal activity of GP and GM extracts against some
human dermatophytes was high. High concentrations of K and Fe were found in the extracts.
GP and GM extracts have great potential as a source for useful antioxidant and antifungal