Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 15, No. 1, 2016, pp. 173-181
Bioline Code: pr16024
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 15, No. 1, 2016, pp. 173-181
© Copyright 2016 - Tropical Journal of Pharmaceutical Research
Determination of 8 Synthetic Food Dyes by Solid Phase Extraction and Reversed-Phase High Performance Liquid Chromatography|
Mazdeh, Fatemeh Zamani; Khorrami, Afshin Rajabi; Moradi-Khatoonabadi, Zhila; EsmaeiliAftabdari, Farideh; Ardekani, Mohammad Reza Shams; Moghaddam, Ghazaleh & Hajimahmoodi, Mannan
Purpose: To develop a simple, selective, and sensitive extraction and assay method for the analysis of
eight food dyes.
Methods: All sulphonated colors were extracted by an NH2-aminopropyl-modified silica SPE cartridge,
and thereafter determined by reverse phase high performance liquid chromatography (RP-HPLC) using
a C18 column with gradient elution of ammonium acetate buffer (pH 6.7), methanol, and acetonitrile.
The analysis was carried out on a UV detector with two optimized method settings within 17 min.
Results: Certificated standard material (CRM) was used to validate the method, and significant
difference was not observed between the results and assigned values. Maximum LOD was 1.154 ppm
at 250 nm for Brilliant blue, and 0.873 ppm for carmoisine. Recovery was 94.2 % for Brilliant blue in jelly
Conclusion: This method was successfully applied to determine colorants in various (30) water-soluble
foods, including fruit flavored drinks, sugar confectionery, sweets, etc.
Synthetic colors; Food; Fruit flavored drinks; Solid phase extraction; RP-HPLC
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