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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 2, 2016, pp. 405-409
Bioline Code: pr16053
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 2, 2016, pp. 405-409

 en Analysis of Chlorogenic Acid Oxidation Pathway in Simulated Enzymatic Honeysuckle Browning System by High Performance Liquid Chromatography and Mass Spectrometry
Luo, Lei; Hao, Yingchao; Jia, Li & Zhu, Wenxue

Abstract

Purpose: To investigate the pathways involved in the oxidation of chlorogenic acid (CA) and phenol metabolism in honeysuckle buds.
Methods: A model that mimics CA oxidation by honeysuckle polyphenol oxidase (PPO) by controlling the reaction temperature or reaction duration was employed, and the resulting products were analyzed by high-performance liquid chromatography followed by mass spectrometry (LC-MS).
Results: The pathway of CA oxidation by PPO in honeysuckle involves CA, hydroxyl - CA, CA-oquinone derivatives, and CA-p-quinone derivatives.
Conclusion: CA oxidation and the enzymatic browning reaction significantly impact the quality of honeysuckle and other fresh agricultural products.

Keywords
Honeysuckle; Chlorogenic acid; Enzymatic browning; Mimic system; Oxidation pathway; High-performance liquid chromatography-mass spectrometry

 
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