To examine the effects of two commercial probiotics (Toyocerin and CloSTAT) on serum
enzyme activities, and hematological and biochemical indices of broiler chickens challenged with
serovars Typhimurium (ST).
The chicks received one of the following treatments at 0 day of age: T1 = control group,
unmedicated diet, unchallenged birds, (negative control); T2 = unmedicated diet + bacterial challenge
(positive control); T3 = medicated diet with neoxyval (0.05 g/kg diet) + bacterial challenge (NEOX); T4 =
toyocerin (1 g/kg diet) + bacterial challenge (Toyocerin); and T5 = CloSTAT (1 g/kg diet) + bacterial
challenge (COLS). Blood samples were withdrawn from 7 selected chicks in each treatment at 7, 21,
28, 35 and 42 days of age, and analyzed for total protein, albumin and globulin concentration, and the
albumin: globulin ratio computed. Glutamate-oxaloacetate transaminase (GOT) and glutatamate
pyruvate transaminase (GPT) levels in serum were measured on days 7 and 42.
The results revealed that albumin (p < 0.001), globulin (p < 0.001) and albumin: globulin ratio
(p < 0.001) were influenced by the time of blood collection. Globulin increased significantly after ST
challenge while albumin decreased significantly. Glutamate-oxaloacetate transaminase (GOT) after ST
challenge was affected by treatment (p < 0.05). Higher levels were obtained from birds which had
received NEOX or positive control, compared to negative control group.
challenge affects serum albumin, globulin and GOT enzyme. The results
obtained suggest that the probiotic, Toyocerin, mitigates the negative effects of Salmonella