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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 3, 2016, pp. 521-526
Bioline Code: pr16070
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 3, 2016, pp. 521-526

 en Anti-neuroinflammatory Effects of Ethanol Extract of Inula helenium check for this species in other resources L (Compositae)
Lee, Sung-Gyu & Kang, Hyun

Abstract

Purpose: To evaluate the in vitro antioxidant and anti-neuroinflammatory activities of Inula helenium check for this species in other resources extract (IHE) against lipopolysaccharide (LPS)-induced production of nitric oxide (NO) by primary microglial cells.
Methods: Cell viability was estimated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyl-tetrazolium bromide (MTT) assay. Antioxidant activity was evaluated using 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay. LPS-stimulated BV-2 microglia were used to study the expression and production of inflammatory mediators, including NO, inducible NO synthase (iNOS) and Interukin-6 (IL-6).
Results: Pretreatment with IHE prior to LPS treatment significantly inhibited excessive production of NO (p < 0.001 at 20, 40, 80 and 100 μg/mL) in a dose-dependent manner, and was associated with downregulation of expression of inducible nitric oxide synthase (iNOS). IHE also suppressed the LPSinduced increase in IL-6 level (p < 0.01 at 40 and 80 μg/mL) in BV-2 cells. The antioxidant activity exhibited by IHE might play a critical role in ameliorating neuroinflammatory processes in LPSstimulated BV-2 microglial cells.
Conclusion: IHE may be beneficial in preventing and treating neurodegenerative and oxidative stressrelated diseases.

Keywords
Inula helenium; DPPH; Neurodegenerative diseases; Anti-inflammatory; Anti-oxidant; Microglial cell

 
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