Purpose: To isolate and identify chemical components of
Phyllostachys prominens
(Poaceae) leaf
extracts, and measure their antioxidant activities.
Methods: Ethanol extracts of
P. prominens leaves were subjected to different chromatographic methods: macroporous resin column chromatography, Sephadex LH-20 column chromatography, and
semi-preparative, reversed-phase (RP) high performance liquid chromatography (HPLC). Plant extract
components were identified by ultraviolet spectroscopy (UV), mass spectrometry (MS), and nuclear
magnetic resonance spectroscopy (NMR). DPPH (1,1-diphenyl-2-picrylhydrazyl) assay was used to
measure the radical scavenging activity of the compounds .
Results: We isolated fourteen compounds including six flavonoids, two lignans, two phenolic
glycosides, a phenolic acid, a phenylpropanoid, a monoterpene glycoside, and amarusine from the
leaves of
Phyllostachys prominens. The DPPH assay showed that eleven compounds (compounds 1, 4,
6, 7, 8, 9, 10, 11, 12, 13, and 14) exhibited radical scavenging activity. (The half maximal inhibitory
concentration ranged from 33.52 to 100.58 μg/mL). The half maximal inhibitory concentration (IC
50)
values of compounds 1, 4, 6 and 7 were 33.52, 40.61, 47.10, and 35.84 μg/mL respectively, while the
IC
50 of the positive control, butylated hydroxytoluene, was 46.32 μg/mL.
Conclusion: Fourteen compounds have been successfully all isolated from
Phyllostachys prominens
for the first time. Eleven of the compounds have radical scavenging activity.
