Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 15, No. 3, 2016, pp. 599-603
Bioline Code: pr16080
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 15, No. 3, 2016, pp. 599-603
© Copyright 2016 - Tropical Journal of Pharmaceutical Research
Synthesis, Characterization and DNA Cleavage of Copper(II) Complex with D,L-Dithiothreitol|
Ismail, T.; Qureshi, M. Z.; Akhtar, N.; Mansoor, Q. & Ismail, M.
Purpose: To study deoxyribonucleic acid (DNA) shearing capability of copper(II) complex of
dithiothreitol (DTT) and to fevaluate its potential application in cancer therapy.
Methods: A parrot green complex was synthesized by grinding copper acetate monohydrate and DTT
in 1:2 molar ratio in a mortar until no fumes of acetic acid were observed. The complex was
characterized using attenuated total reflectance-Fourier transform infra-red (ATR-FTIR), and x-ray
diffraction (XRD) techniques. Further information was also collected through Karl Fischer titration,
thermogravimetric analysis (TGA) and (magnetic moment. Cleavage of DNA was determined by
agarose gel electrophoresis. The gel was then stained, analyzed and photographed under ultraviolet
Results: ATR-FTIR confirmed the formation of copper(II) complex with DTT by binding through thiol
group based on the disappearance of the thiol (-SH) stretching peak at 2545 cm-1. The crystalline
structure was elucidated by a sharp intense peak at 38.520 in XRD spectrum while the octahedral
geometry of complex was inferred from a magnetic moment of 1.72 B.M. The results for water content
obtained by Karl Fischer titration and TGA revealed that water molecules are not part of the coordination
sphere of the complex. Cleavage study of DNA showed that the complex completely sheared
the circular DNA compared to pure DTT.
Conclusion: Solvent free synthesis of Copper(II)-DTT complex has been successfully achieved, and an
anhydrous complex with octahedral geometry obtained. The complex has a greater potential to shear
DNA molecule than pure DTT.
DNA shearing; Copper(II) complex; Dithiothreitol; Attenuated total reflectance-Fourier transform infra-red; Karl Fischer titration; Magnetic moment
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