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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 3, 2016, pp. 605-611
Bioline Code: pr16081
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 3, 2016, pp. 605-611

 en Simultaneous Determination of Famotidine and Flurbiprofen by High Performance Liquid Chromatography
Hanif, Muhammad; Nazer, Nida; Chaurasiya, Vesh & Zia, Usman

Abstract

Purpose: To develop a selective, sensitive and accurate simultaneous High Performance Liquid Chromatography (HPLC) method for the analysis of flurbiprofen and famotidine tablet dosage form and excipients.
Methods: A simultaneous method for the determination of the two drugs was employed. The assay consisted of isocratic elution of flurbiprofen and famotidine in prepacked Column RT 250-4.6 Purospher® STAR RP-18 end-capped (5 μm), using a mobile phase composed of acetonitrile and phosphate buffer (pH 3.7) in a ratio of 1:1, respectively, as well as an ultraviolet (UV) detector. Flurbiprofen and famotidine were both detected at 265 nm at a flow rate of 1 ml/min following the guidelines of International Conference of Harmonization (ICH).
Results: A sensitive and linear range of 10 to 100 ppm with 0.999 coefficient of correlation for famotidine and flurbiprofen were observed. Blank and placebo showed no interference with the peak retention time of flurbiprofen and famotidine. Recovery of 50 mg famotidine was 99.61 % after 12 h and 99.76 % after 7 days and for flurbiprofen 20 mg it were 99.60 % after 24 h and 99.85 % after 7 days.
Conclusion: Good precision, recovery and accuracy data indicate the reliability of the proposed method. The method may also be suitable for use in bioequivalence studies.

Keywords
Flurbiprofen; Famotidine; Retention time; Limit of detection; Limit of quantification

 
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