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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 4, 2016, pp. 793-798
Bioline Code: pr16106
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 4, 2016, pp. 793-798

 en Phytochemical screening and evaluation of cytotoxicity of stem bark extracts of Genus dolichocarpa check for this species in other resources and Duguetia chrysocarpa check for this species in other resources (Annonaceae)
da Silva Pinheiro, Reísa; Rabelo, Suzana Vieira; de Oliveira, Ana Paula; Guimarães, Amanda Leite; de Moraes-Filho, Manoel Odorico; da Costa, Marcília Pinheiro; do Ó Pessoa, Cláudia; Carneiro Lúcio, Ana Sílvia Suassuna & da Silva Almeida, Jackson Roberto Guedes


Purpose: To investigate the phytochemistry and cytotoxic activity of stem bark extracts from Genus dolichocarpa check for this species in other resources and Duguetia chrysocarpa check for this species in other resources - two species of the Annonaceae family.
Methods: The crude ethanol bark extracts (EtOH) of the plants were obtained by maceration. The crude extracts were suspended in a mixture of methanol (MeOH) and water (H2O) (proportion 3:7 v/v) and partitioned with hexane, chloroform (CHCl3) and ethyl acetate (AcOEt) in ascending order of polarity to obtain the respective fractions. The extracts were evaluated on thin layer chromatography (TLC) plates of silica gel to highlight the main groups of secondary metabolites. Cytotoxicity was tested against human tumor cell lines - OVCAR-8 (ovarian), SF-295 (brain) and HCT-116 (colon) - using 3- (4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay.
Results: The screening results demonstrated that all the extracts were positive for the presence of flavonoids and tannins. The presence of alkaloids also was detected in some extracts. The hexane extract of A. dolichocarpa showed the strongest cytotoxicity against HCT-116 with cell growth inhibition of 89.02 %.
Conclusion: The findings demonstrate for the first time the cytotoxic activity of the extracts of A. dolichocarpa and D. chrysocarpa, thus providing some evidence that plants of the Annonaceae family are a source of active secondary metabolites with cytotoxic activity.

Annonaceae; Duguetia chrysocarpa; Ovarian; Anaxagorea dolichocarpa; Anti-cancer agents; brain and colon cell lines

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