To study the prevalence of resistant strains of Staphylococcus aureus
isolated from surfaces, beds and
various equipment of an Iranian hospital emergency ward.
Two hundred swab samples were collected from the surfaces, beds, trolleys, surgical
equipment and diagnostic medical devices in emergency ward. Samples were cultured and those that
were S. aureus-positive were confirmed using polymerase chain reaction (PCR). Antimicrobial
resistance pattern was analyzed using disk diffusion method.
Nine of 200 samples (4.5 %) collected were positive for S. aureus. Surfaces (8.8 %), beds (5
%) and trolleys (5 %) were the most commonly contaminated. S. aureus isolates exhibited varying
levels of resistance against antibiotics with the following being the highest: tetracycline (88.8 %),
penicillin (88.8 %) and ampicillin (77.7 %). The prevalence of resistance against methicillin, oxacillin and
azithromycin were 44.4, 33.3 and 33.3 %, respectively. There was no pattern of resistance against
Efficient disinfection of surfaces, beds, trolleys and surgical instruments should be
performed periodically to reduce colonization of resistant strains of S. aureus in various areas of
emergency health care centers.