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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 5, 2016, pp. 1039-1047
Bioline Code: pr16139
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 5, 2016, pp. 1039-1047

 en Anti-biofilm and antimicrobial activity of Mentha pulegium check for this species in other resources L essential oil against multidrug-resistant Acinetobacter baumannii check for this species in other resources
Tutar, Uğur; Çelik, Cem; Karaman, İsa; Ataş, Mehmet & Hepokur, Ceylan

Abstract


Purpose: To investigate the antimicrobial and anti-biofilm activities of essential oil from Mentha pulegium check for this species in other resources L. (EOMP) on multi-drug resistant (MDR) isolates of A. baumannii check for this species in other resources , as well as its phytochemical composition, antioxidant properties and cytotoxic activity.
Methods: The phytochemical composition of EOMP was analyzed by gas chromatography, while its antimicrobial activities were determined by disc diffusion and broth micro-dilution methods. Minimal biofilm inhibition concentration (MBIC) and minimal biofilm eradication concentration (MBEC) tests were used for assessment of its anti-biofilm properties. Viability in the biofilm was studied using 2,3-bis (2- methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay, while colorimetric assay was used to assess its cytotoxicity on L929 cells.
Results: D-isomenthone, pulegone, isopulegone, menthol and piperitenone were the major components of the plant extract. EOMP produced > 22 mm inhibition zone for the isolates, with minimum inhibitory concentration (MIC) and MBIC of 0.6 - 2.5 and 0.6 - 1.25 μL/mL, respectively, while MBEC was ≥ 10 μL/msL. EOMP damaged biofilm structures formed by A. baumannii strains at MIC by 26 – 91 %.
Conclusion: These results suggest that EOMP contains agents that may be useful in the development of new drugs against A. baumannii infections.

Keywords
Mentha pulegium; Anti-biofilm; Multi-drug resistance; Essential oil; Antimicrobial activity; A. baumannii

 
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