search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 5, 2016, pp. 1047-1053
Bioline Code: pr16140
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 5, 2016, pp. 1047-1053

 en Prevalence of Listeria check for this species in other resources species in some foods and their rapid identification
Yehia, Hany M.; Ibraheim, Shimaa M. & Hassanein, Wesam A.

Abstract


Purpose: To investigate the occurrence of Listeria check for this species in other resources spp., (particularly L. monocytogenes check for this species in other resources ), in different foods and to compare diagnostic tools for their identification at species level.
Methods: Samples of high protein foods such as raw meats and meat products and including beef products, chicken, fish and camel milk were analysed for the presence of Listeria spp. The isolates were characterised by morphological and cultural analyses, and confirmed isolates were identified by protein profiling and verified using API Listeria system. Protein profiling by SDS-PAGE was also used to identify Listeria spp.
Results: Out of 40 meat samples, 14 (35 %) samples were contaminated with Listeria spp., with the highest incidence (50 %) occurring in raw beef products and raw chicken. Protein profiling by SDSPAGE was used to identify Listeria spp. The results were verified with API Listeria system. Approximately 25 % of the identified isolates were Listeria seeligeri check for this species in other resources , Listeria welshimeri check for this species in other resources , and Listeria grayi check for this species in other resources (three positive samples), while 16.66 % of the isolates were Listeria monocytogenes (two positive samples); 16.6 % of the isolates were Listeria innocua check for this species in other resources (two positive samples), while 8.3 % of the isolates were Listeria ivanovii check for this species in other resources (one positive sample).
Conclusion: High protein foods contain different types of Listeria species; whole-cell protein profiles and API Listeria system can help in the identification of Listeria at the species level.

Keywords
Listeria spp; Api Listeria; High protein food; Protein profile

 
© Copyright 2016 - Tropical Journal of Pharmaceutical Research
Alternative site location: http://www.tjpr.org

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2022, Site last up-dated on 19-Jan-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil