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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 6, 2016, pp. 1243-1249
Bioline Code: pr16165
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 6, 2016, pp. 1243-1249

 en Antitumor effect and mechanism of action of polysaccharides extracted from Polygonum perfoliatum check for this species in other resources L whole plant in human lung carcinoma A549 cell line
Lai, Xi-xi & Li, Yu-ping

Abstract

Purpose: To optimize the extraction conditions of polysaccharides from Polygonum perfoliatum check for this species in other resources L. (PSDP) and to evaluate their anti-tumor activities on A549 cell line.
Methods: Extraction of PSDP was optimized using Box-Behnken design (BBD). Three factors of response surface methodology (RSM) including extraction time, ratio of water to raw material and number of extractions were employed to optimize the yield of PSDP. The cytotoxic effect of PSDP on human lung carcinoma A549 cell line was evaluated in vivo, while its effects on expressions of caspase-3, caspase-9, Bcl-2 and Bax were determined by western blot assay. Result: BBD was significant and applicable to PSDP extraction. Based on the contour plots, response surface plots and variance analysis, it predicted that the optimum conditions for PSDP extraction were: 1.58 h (extraction time); 30.18 mL/g (ratio of water to raw material); and 2.02 (number of extractions). PSDP had significant inhibitory effect on the growth of A549 cells in a concentration- and timedependent manner (p < 0.05). After treatment with PSDP, caspase-3, caspase-9 and Bax were significantly up-regulated (p < 0.05), whereas Bcl-2 was down-regulated, all concentration-dependently.
Conclusion: RSM analysis is an appropriate method to optimize PSDP extraction. The results also indicate that PSDP has significant anti-tumor effect against A549 cells, most likely via inducing mitochondria-mediated apoptosis.

Keywords
Polygonum perfoliatum; Polysaccharides; Anti-tumor effect; Human lung carcinoma; Mitochondria-mediated apoptosis

 
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