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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 15, No. 6, 2016, pp. 1259-1265
Bioline Code: pr16167
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 6, 2016, pp. 1259-1265

 en Bioprospecting for culturable actinobacteria with antimicrobial properties isolated from rivers in Colombian Orinoquia
Pastrana-Camacho, Natalia; Suárez, Zulma; Acosta-González, Alejandro; Arango, Carolina; Haltli, Brad; Correa, Hebelin; Kerr, Russel; Duque, Carmenza & Díaz, Luis Eduardo


Purpose: To Isolate and characterize Actinobacteria with antimicrobial activity from Guaviare River (Colombia).
Methods: Water and sediment samples were collected from Guaviare River. Direct plating, heat and CaCO3 methods were used to isolate Actinobacteria. Six bacterial strains were tested using T-Streak method: Escherichia coli ATCC 23724, Staphylococus aureus ATCC 25923, Acinetobacter baumannii ATCC 19606, Bacillus subtilis ATCC 21556, Klebsiella pneumoniae ATCC 700603, Chromobacterium violaceum ATCC 31532. Strains of Fusarium sp. H24, Trichoderma harzianum H5 and Colletotrichum gloeosporioides were tested using Kirby-Bauer method. Isolates with high antimicrobial activity were selected for further taxonomic identification.
Results: A total of 374 actinobacteria isolates were obtained. Seven isolates exhibited high antimicrobial activity (p < 0.05) and were confirmed as members of Streptomycetaceae family. Of these, three isolates showed differential phenotypic and genotypic profiles, indicating that they may represent new species.
Conclusions: To date, this is the first study of this type in Colombian Orinoquia and indicates that this promising source of Actinobacteria from aquatic sediments with the ability to produce antimicrobial secondary metabolites.

Actinobacteria; Antimicrobial activity; Secondary metabolites; Streptomycetaceae; Phenotypic and genotypic profiles; Colombian Orinoquia

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