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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 7, 2016, pp. 1431-1438
Bioline Code: pr16189
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 7, 2016, pp. 1431-1438

 en Antibacterial and cytotoxic properties of isoprenoids from the red sea soft coral, Lobophytum check for this species in other resources sp
Al-Footy, Khalid O.; Alarif, Walied M.; Zubair, Muhammad S.; Ghandourah, Mohamed A. & Aly, Magda M.

Abstract

Purpose: To evaluate the antibacterial and cytotoxic activities of the secondary metabolites of Lobophytum check for this species in other resources sp.
Methods: Maceration with methanol: chloroform (1:1) was applied to extract the coral material. Chromatographic and spectroscopic techniques were employed for fractionation, isolation and elucidation of pure compounds. Antibacterial activities were performed by well diffusion method against three Gram-positive and four Gram-negative bacteria. Brine shrimp lethality test was employed to predict toxicity, while antitumor activity were tested by 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide (MTT) method against Ehrlich carcinoma cells.
Results: Four sesquiterpenes, one cembranoid type diterpenes and two steroids were isolated. 1 exhibited significant antibacterial activity against four tested bacteria ( P. aeruginosa check for this species in other resources , S. aureus check for this species in other resources , S. epidermis check for this species in other resources , and S. pneumonia check for this species in other resources ) with MIC value of 15 μg/mL. Moreover, 1 showed high diameter zone of inhibition ranging from 16 - 18 mm against test bacteria. Compounds 4 and 5 displayed moderate antibacterial activity against all test bacteria with inhibition zone diameter (IZD) ranging from 11 – 15 mm and MIC values of 30 μg/mL. 2, 3, 6 and 7 exhibited weak antibacterial activity (IZD, 7 - 11 mm; MIC ≥ 30 μg/mL). In addition, only diterpene compound (4) showed high toxicity against A. Salina and antitumor activity against Erhlich carcinoma cells with the LD50 of 25 and 50 μg/mL, respectively.
Conclusion: This study reveals the strong antibacterial activity of sesquiterpene alismol (1) and the potential antibacterial and antitumor activity of cembranoid type diterpene, cembrene A (4).

Keywords
Soft coral; Lobophytum sp; Red Sea; Antibacterial; Cytotoxicity; Sesquiterpene Alismol; Cembranoid; Diterpene; Cembrene

 
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