Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 15, No. 8, 2016, pp. 1613-1619
Bioline Code: pr16211
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 15, No. 8, 2016, pp. 1613-1619
© Copyright 2016 - Tropical Journal of Pharmaceutical Research
Anti-platelet aggregation of mixtures of betulinic oleanolic and maslinic acids and derivatives from medicinal plants|
Osunsanmi, Foluso O.; Oyinloye, Babatunji E.; Mosa, Rebamang A.; Ikhile, Monisola I.; Ngila, J. Catherine; Shode, Francis O.; Singh, M. & Opoku, Andy R.
Purpose: To evaluate the antiplatelet aggregation and cytotoxic potential of betulinic acid (BA),
oleanolic acid (OA), maslinic acid (MA) and their derivatives (3-β-acetyloleanolic acid (OAA) and 3-β-
acetylbeutulinic (BAA) from medicinal plants.
Methods: The compounds were characterized by nuclear magnetic resonance (NMR, both carbon 13
and hydrogen 1) (NMR), infra-red (FTIR) and mass spectroscopy (MS). The platelet aggregation
inhibitory activities of the compounds (1, 3, 5 and 10 mg/ml) were investigated separately on adenosine
diphosphate (ADP) and thrombin-induced rat platelet aggregation. Cytotoxicity studies were carried out
on human embryonic kidney (HEK293) and hepatocellular carcinoma (HEPG2) cell lines using 3, 4, 5-
Results: The compounds significantly (p < 0.05) inhibited platelet aggregation in a dose-dependent
manner on thrombin and ADP agonist. BAA/OAA showed the highest activity on both agonists with IC50
of 2.86 and 3.05 mg/mL respectively. BAA/OAA also showed better antiplatelet activity than aspirin (IC50
of 6.45 and 7.36 mg/mL, respectively). In addition the compound (BA/OA, BAA/OAA and MA/OA)
exhibited low cytotoxic effect on both HEK293 cells (IC50: 724.43, 269.08 and 407.89 mg/mL
respectively) and HEPG2 (IC50: 585.38, 499.78 and 499.78 mg/mL, respectively).
Conclusion: BAA/OA:A demonstrate the best antiplatelet potential and low cytotoxicity of in all the tests,
and therefore can serve as safer antiplatelet agents.
Platelet aggregation; Agonist; Aspirin; Betulinic Acid; Oleanolic Acid; Maslinic Acid; Cytotoxicity
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