To study the vacA genotype status of H. pylori
isolated from some Iranian food items.
Three hundred assorted samples of fish, ham, chicken, vegetable and meat sandwiches, and
minced meat were purchased and tested using culture method. Those that were H. pylori
analyzed for presence of vacA genotypes using polymerase chain reaction (PCR).
Sixty out of 300 (20 %) food samples were positive for H. pylori
. Vegetable sandwich (45 %),
minced meat (32 %) and meat sandwich (20 %) were the most commonly contaminated. The most
commonly detected genotypes in the meat-based foods, viz, vegetable sandwich and ready to eat fish,
were vacA s1a, vacA m1a and vacA m2, respectively. The most commonly detected combined
genotypes were s1am2 (45 %), s1am1a (40 %) and m1am2 (35 %).
The presence of similar genotypes in H. pylori
strains of foods and those of human clinical
samples suggest that contaminated foods may be the source of bacteria for humans.