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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 8, 2016, pp. 1631-1636
Bioline Code: pr16213
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 8, 2016, pp. 1631-1636

 en Genotyping of vacA alleles of Helicobacter pylori check for this species in other resources strains recovered from some Iranian food items
Ghorbani, Fatemeh; Gheisari, Elham & Dehkordi, Farhad Safarpoor

Abstract

Purpose: To study the vacA genotype status of H. pylori check for this species in other resources isolated from some Iranian food items.
Methods: Three hundred assorted samples of fish, ham, chicken, vegetable and meat sandwiches, and minced meat were purchased and tested using culture method. Those that were H. pylori-positive were analyzed for presence of vacA genotypes using polymerase chain reaction (PCR).
Results: Sixty out of 300 (20 %) food samples were positive for H. pylori. Vegetable sandwich (45 %), minced meat (32 %) and meat sandwich (20 %) were the most commonly contaminated. The most commonly detected genotypes in the meat-based foods, viz, vegetable sandwich and ready to eat fish, were vacA s1a, vacA m1a and vacA m2, respectively. The most commonly detected combined genotypes were s1am2 (45 %), s1am1a (40 %) and m1am2 (35 %).
Conclusion: The presence of similar genotypes in H. pylori strains of foods and those of human clinical samples suggest that contaminated foods may be the source of bacteria for humans.

Keywords
Helicobacter pylori; VacA genotypes; Genotyping; Food items

 
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