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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 8, 2016, pp. 1707-1713
Bioline Code: pr16224
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 8, 2016, pp. 1707-1713

 en In vitro evaluation of inhibitory effect of Phoenix dactylifera check for this species in other resources bark extract on rat lipid peroxidation and blood hemolysis
Siahpoosh, Amir & Soleimani, Inas


Purpose: To determine the polyphenolic content of P. dactylifera check for this species in other resources L. (date palm) bark extract and to investigate its in vitro inhibitory effects on lipid peroxidation in the brain, liver, and kidney tissues of rat, as well as on blood hemolysis in rats.
Methods: P. dactylifera L. barks were collected from Ahvaz, (Khuzestan, Iran). Methanolic extract of the P. dactylifera barks were prepared using maceration method. Total phenolic, flavonoid and proanthocyanidin contents were determined by colorimetric methods. The effects of the extract were investigated on thiobarbituric acid reactive substances formation in Fe2+/ascorbate induced-lipid peroxidation in brain, liver and kidney tissues of rats. Furthermore, the inhibitory effects of the extracts on erythrocyte hemolysis caused by 2,2'-Azobis (2-amidinopropane) dihydrochloride (AAPH) were evaluated.
Results: Total phenolic, flavonoids and proanthocyanidins contents per each g dry extract of P. dactylifera L. were 50.7 mg tannic acid, 10.38 mg rutin, and 5.45 mg cyanidin, respectively, while halfmaximal inhibitory concentration (IC50) for inhibition of lipid peroxidation in brain, liver, and kidney tissues of rats was 3150.71, 1941.45, and 1546.01 μg/mL, respectively, and for the inhibition of erythrocyte hemolysis, it was 472.41 μg/mL.
Conclusion: The findings of this study indicate significant anti-lipid peroxidation and anti-hemolytic effects of the bark extract. Therefore, the extract can potentially be used for the in vivo treatment of diseases associated with lipid peroxidation such as cancers and Alzheimer\'s disease, but further studies are required.

Phoenix dactylifera; Date palm; Lipid peroxidation; Antioxidant; Erythrocyte hemolysis

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