To investigate the anti-tumor effects of polysaccharides from Curculigo orchioides
cervical cancer and the possible mechanisms involved.
A Box–Behnken design (BBD) was employed to optimize extraction conditions for PDC. The
anti-tumor effect of PDC on cervical cancer was investigated in vivo
in mice injected with Hela cells. The
parameters measured were tumor volume and weight. In vitro
anti-tumor effects of PDC were assessed
by measuring expressions of caspase-3, caspase-9 and P53 proteins in Hela cells via ELISA assay.
Thymus and spleen indices were calculated for assessment of PDC effect on immune function.
The optimum extraction conditions predicted by the response surface methodology (RSM)
were: extraction time = 1.58 h, ratio-of-water-to-sample = 30.05 mL/g and extraction number = 1.95.
PDC showed significant anti-tumor effect on cervical cancer in mice. It significantly increased thymus
and spleen indices in mice; and significantly up-regulated expressions of caspase-3, caspase-9 and
P53 proteins in HeLa cells.
PDC has significant anti-tumor effect on cervical cancer in vivo
and in vitro
, most probably
through mechanisms involving enhancement on immune function and induction of apoptosis.