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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 8, 2016, pp. 1731-1737
Bioline Code: pr16227
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 8, 2016, pp. 1731-1737

 en Anti-tumor effect of polysaccharides from rhizome of Curculigo orchioides check for this species in other resources Gaertn on cervical cancer
Xia, Ling-fang; Liang, Shan-hui; Wen, Hao; Tang, Jia & Huang, Yan

Abstract

Purpose: To investigate the anti-tumor effects of polysaccharides from Curculigo orchioides check for this species in other resources (PDC) on cervical cancer and the possible mechanisms involved.
Methods: A Box–Behnken design (BBD) was employed to optimize extraction conditions for PDC. The anti-tumor effect of PDC on cervical cancer was investigated in vivo in mice injected with Hela cells. The parameters measured were tumor volume and weight. In vitro anti-tumor effects of PDC were assessed by measuring expressions of caspase-3, caspase-9 and P53 proteins in Hela cells via ELISA assay. Thymus and spleen indices were calculated for assessment of PDC effect on immune function.
Results: The optimum extraction conditions predicted by the response surface methodology (RSM) were: extraction time = 1.58 h, ratio-of-water-to-sample = 30.05 mL/g and extraction number = 1.95. PDC showed significant anti-tumor effect on cervical cancer in mice. It significantly increased thymus and spleen indices in mice; and significantly up-regulated expressions of caspase-3, caspase-9 and P53 proteins in HeLa cells.
Conclusion: PDC has significant anti-tumor effect on cervical cancer in vivo and in vitro, most probably through mechanisms involving enhancement on immune function and induction of apoptosis.

Keywords
Curculigo orchioides; Polysaccharides; Cervical cancer; HeLa cells; Apoptosis

 
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