Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 15, No. 8, 2016, pp. 1759-1765
Bioline Code: pr16231
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 15, No. 8, 2016, pp. 1759-1765
© Copyright 2016 - Tropical Journal of Pharmaceutical Research
Quantitative determination of alliin in dried garlic cloves and products by high-performance thin-layer chromatography|
Siddiqui, Nasir A.; Mothana, Ramzi A. & Alam, Perwez
Purpose: To standardize the garlic samples and its products for alliin contents.
Methods: A direct high-performance thin-layer chromatographic (HPTLC) method was developed to
determine alliin in Chinese (CG) and Indian garlic (IG) and two other marketed products from USA and
UK, respectively. Scanning and quantification were performed at 205 nm. Furthermore, chromatography
was performed on pre-coated HPTLC plates with the solvent mixture, n – hexane:ethyl acetate (29:1
v/v), as the mobile phase. In addition, the parameters suggested by International Conference on
Harmonization for analytical procedures were considered to validate the proposed method.
Results: The system gave a compact spot of alliin at RF = 0.19 ± 0.01 The linear regression data for
the calibration plots showed a good linearity (r2 = 0.996) in the concentration range of 200 - 1600 ng.
Linear regression equation was represented by Y = 1.792x + 182.855, while LOD and LOQ values were
40.42 ngband-1 and 111.72 ng.band-1, respectively. The method showed excellent accuracy with
recovery of 98.20 – 99.10 % and good precision with RSD of 1 - 2.65 %.
Conclusion: The proposed method is selective, sensitive and accurate for the determination of alliin in
garlic and its products. It is also evident from the results obtained that raw Indian garlic has higher alliin
content than Chinese garlic.
Garlic; HPTLC; Alliin; Hypercholesteremia; Quantification
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