Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 15, No. 9, 2016, pp. 1847-1852
Bioline Code: pr16243
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 15, No. 9, 2016, pp. 1847-1852
© Copyright 2016 - Tropical Journal of Pharmaceutical Research
Expression of Oct-4 in oncogenic miR-155-positive oral squamous carcinoma cells|
Jiang, Jun-Song; Han, Hua; Zhu, Jia & Wang, Qing
Purpose: To identify effective molecular diagnostic methods for oral squamous cell carcinoma (OSCC)
to facilitate treatment of the disease in its initial stages.
Methods: To identify molecular markers, OSCC tissue samples were collected from cancer patients
and healthy controls. CD44+ cells were sorted using quantitative real-time polymerase chain reaction
(qRT-PCR). Immunohistochemistry and immunostaining experiments were performed to identify
markers for OSCC.
Results: The qRT-PCR data confirmed the presence of oncogenic miR-155 in the OSCC samples. The
immunohistochemical and immunostaining results confirmed the expression of Oct-4, an important
target for the early diagnosis of OSCC, in oncogenic miR-155-positive OSCCs.
Conclusion: Detection of the expression of miR-155 and Oct-4, which are key molecular markers, may
be useful in improving the early diagnosis of OSCC.
CD44; oral squamous cell carcinoma; oncogenic miR-155; Oct-4
Alternative site location: http://www.tjpr.org