Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 15, No. 9, 2016, pp. 1897-1901
Bioline Code: pr16250
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 15, No. 9, 2016, pp. 1897-1901
© Copyright 2016 - Tropical Journal of Pharmaceutical Research
Anti-osteoporosis activity of Astragalus membranaceus Bunge extract in experimental rats|
Li, Hongbo; Nie, Daqing; Wang, Chengwu; Fang, Jiaming & Li, Da
Purpose: To investigate the anti-osteoporosis effect of Astragalus membranaceus (Fisch.) Bunge.
extract (AMBE) in experimental rats.
Method: Female Sprague-Dawley rats were randomly divided into six groups: control group,
ovariectomy (OVX) with vehicle group, OVX with 17β-estradiol (E2, 25 μg/kg/day) group, and OVX with
AMBE doses (60, 120 and 240 mg/kg/day) groups. Daily oral administration of AMBE or E2 was started
4 weeks after OVX and lasted for 16 weeks. The bone mineral density (BMD) of L4 vertebrae and right
femurs was evaluated. The length of each femur was measured with a micrometer, and the center of
diaphysis was determined. Three representative L4 vertebrae were selected to evaluate trabecular
microarchitecture. Serum alkaline phosphatase (ALP), urinary calcium (U-Ca), urinary phosphorus (UP),
urinary creatinine (Cr) and osteocalcin (OC) levels were measured.
Results: AMBE dose-dependently inhibited the bone mineral density (BMD) reduction of L4 vertebrae
(0.27 ± 0.03 g/cm2, p < 0.05) and femurs (0.23 ± 0.03 g/cm2, p < 0.05) caused by OVX and prevented
the deterioration of trabecular microarchitecture (p < 0.05), which were accompanied by a significant
decrease in skeletal remodeling (p < 0.05) as evidenced by the lower levels of bone turnover markers. A
higher dosage of AMBE treatment (240 mg/kg/day) increased U-Ca/Cr (0.27 ± 0.03 mmol/mmol), ALP
(137.23 ± 16.72 U/L), U-P/Cr (4.18 ± 0.27 mmol/mmol) and OC (8.47 ± 0.26 mmol/L) levels (both p <
Conclusion: The findings of this study indicate that AMBE prevents OVX-induced osteoporosis in rats.
Astragalus membranaceus (Fisch.) Bunge; Osteoporosis; Ovariectomy; Bone mineral density
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