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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 9, 2016, pp. 1915-1921
Bioline Code: pr16253
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 9, 2016, pp. 1915-1921

 en In vivo analgesic activity and safety assessment of Vitis vinifera check for this species in other resources L and Punica granatum check for this species in other resources L fruits extracts
Zeghad, Nadia; Madi, Aicha; Helmi, Sihem & Belkhiri, Abdelmalik

Abstract

Purpose: To investigate the analgesic properties of fruit extracts of Vitis vinifera check for this species in other resources (grape) and Punica granatum check for this species in other resources (pomegranate) in Albino mal mice.
Methods: The analgesic activity of fruit extracts of V. vinifera and P. granatum check for this species in other resources were examined in vivo using thermal stimulus assays (i.e., tail immersion and hot plate) and acetic acid-induced writhing test using acetylsalicylic acid (0.1 g/kg, per os) as standard. The extracts were administered orally in doses of 1.0, 2.0 and 3.0 g/kg.
Results: In acetic acid writhes test, both fruit extract pretreatments (1.0, 2.0 and 3.0 g/kg, per os) significantly decreased the number of writhes (p < 0.0001) in a dose-dependent manner compared to control. The Index of Pain Inhibition (IPI) values following V. vinifera extract treatments were 36.52 % (1.0 g/kg), 66.67 % (2.0 g/kg) and 89.71 % (3.0 g/kg) which were significantly different from those for P. granatum extracts (45.39 %, 1.0 g/kg), 70.93 %, 2.0 g/kg) and 86.88 %, 3.0 g/kg) at equivalent doses of 2.0 and 3.0 g/kg of the extracts The fruit extracts of both species increased the reaction latency time. In tail-immersion assay, only the fruit extract of P. granatum significantly increased the response to heat stimulus at doses of 2.0 g/kg (p < 0.05).
Conclusion: The hydroalcohol fruit extracts of P. granatum and V. vinifera have potential analgesic effects. Further studies are needed to determine the active component responsible for this effect.

Keywords
Vitis vinifera; Punica granatum; Analgesic activity; Tail immersion test; Hot plate test; mouse writhings inhibition assay

 
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