Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 15, No. 9, 2016, pp. 1947-1953
Bioline Code: pr16258
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 15, No. 9, 2016, pp. 1947-1953
© Copyright 2016 - Tropical Journal of Pharmaceutical Research
Prevalence of AmpC β-lactamase among Gram-negative bacteria recovered from clinical specimens in Benin City, Nigeria|
Ogefere, Helen O.; Osikobia, James G. & Omoregie, Richard
Purpose: Infections caused by AmpC-positive bacteria results in high patient morbidity and mortality
making their detection clinically important as they cannot be detected in routine susceptibility testing.
This study aim to determine the prevalence of AmpC β-lactamase among Gram negative bacteria
recovered from clinical specimens in Benin City, Nigeria.
Methods: A total of 256 consecutive and non-repetitive Gram negative bacteria were recovered from
various clinical specimens. The prevalence of AmpC β-lactamase was determined using a combination
of disc antagonism test and cefoxitin-cloxacillin inhibition test. Disc susceptibility test was performed on
all isolates using standard techniques.
Results: Cefoxitin-cloxacillin inhibition test detected more AmpC β-lactamase than other tests. The
prevalence of AmpC β-lactamase did not differ significantly between both genders and between inpatients
and out-patients (p>0.05). Isolates recovered from sputum had significantly higher prevalence
of AmpC β-lactamase producers compared with isolates from other clinical specimens (p=0.0484). The
prevalence of AmpC production was significantly higher among isolates of Pseudomonas aeruginosa
than other isolates (p = 0.0085). Isolates that produced AmpC β-lactamase were more susceptible to
the test cephalosoprins.
Conclusion: An overall prevalence of AmpC β-lactamase (15.23 %) was observed in this study.
Pseudomonas aeruginosa was the most prevalent producer of AmpC enzymes. Prudent use of
antibiotics is advocated.
AmpC β-lactamase producers; AmpC β-lactamase producers; AmpC β-lactamase producers; Pseudomonas aeruginosa; routine susceptibility testing
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