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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 10, 2016, pp. 2113-2119
Bioline Code: pr16280
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 10, 2016, pp. 2113-2119

 en Anti-leishmanial and cytotoxic activities of extracts from three Pakistani plants
Iqbal, Kashif; Iqbal, Javeid; Umair, Muhammad; Farooq, Umar; Iqbal, Muhammad Mohsin; Qamar, Sumbal & Bashir, Mehreen

Abstract

Purpose: To evaluate the in vitro and in vivo anti-leishmanial and cytotoxic activities of extracts of different parts of Lawsonia Inermis check for this species in other resources , Morus nigra check for this species in other resources and Ziziphus mauritiana check for this species in other resources .
Methods: The methanol extracts of all three plant materials at concentrations of 10 - 100 μg/mL were tested for their in vitro anti-leishmanial effects on L. tropica check for this species in other resources KWH23 promastigotes for 24 - 48 h, relative to negative control and amphotericin-B (standard drug). For in vivo anti-leishmanial activity, the extracts were tested against L. tropica-infected albino mice, while cytotoxicity was investigated against mammalian cells (lymphocytes).
Results: For Lawsonia Inermis leaves, mean inhibition of extracellular promastigotes at 10, 25, 50 and 100 μg/mL after 48 h were 98.2 ± 0.06, 98.75 ± 1.09, 99.31 ± 0.00 and 100.00 ± 0.00 %, respectively. After 8 weeks, mean lesion size decreased from 0.8 ± 0.2 mm to 0.3 ± 0.1 mm (p < 0.01), and cure at 150 mg/kg against intracellular amastigotes in albino mice was 97.02 % (95 % CI = 96.14 - 98.10). IC50 for Lawsonia inermis leaf extract was 12.22 μg/mL (95 % CI = 11.54 - 13.84) against lymphocytes.
Conclusion: The results obtained in this study show that Lawsonia Inermis leaf is safe and possesses potent anti-leishmanial activity.

Keywords
Leishmania tropica; Lawsonia inermis; Morus Nigra; Ziziphus Mauritiana; Anti-leishmania; Amastigotes; Lymphocytes

 
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