Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 15, No. 10, 2016, pp. 2165-2172
Bioline Code: pr16286
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 15, No. 10, 2016, pp. 2165-2172
© Copyright 2016 - Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria.
Anti-depressant-like effect of curculigoside isolated from Curculigo orchioides Gaertn root|
Wang, Jing; Zhao, Xiao-Li & Gao, Lan
Purpose: To investigate the anti-depressant-like activity of curculigoside from Curculigo orchioides
Gaertn and its underlying mechanism(s).
Methods: Antidepressant-like activity was determined in mice through forced swimming test (FST), tail
suspending test (TST), and open field test (OFT). Mechanism of action was investigated by measuring
levels of dopamine (DA), norepinephrine (NE), and 5-hydroxytryptamine (5-HT) in chronic mild stress
(CMS) rats using high-performance liquid chromatography-electron capture detector (HPLC-ECD).
Western blotting was used to investigate the effect of curculigoside on the expression of brain-derived
neurotrophic factor (BDNF) protein in rats.
Results: In FST and TST, treatment of mice with curculigoside (10, 20, 40 mg/kg, p.o.) significantly
reduced immobility time, which was, however, unaffected by locomotor activity when assessed in the
OFT. The treatment led to significant increases in DA, NE and 5-HT, and up-regulation of BDNF protein
expression in the hippocampus of the CMS rats.
Conclusion: These results demonstrate that curculigoside exerts significant anti-depressant-like activity
on mice, the mechanism of which involves increase in the levels of DA, NE, 5-HT, and up-regulation of
BDNF expression. Thus, curculigoside can be considered a potential drug candidate for the treatment of
Curculigo orchioides Gaertn; Curculigoside; Antidepressant-like activity; Behavioral studies
Alternative site location: http://www.tjpr.org