Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 15, No. 10, 2016, pp. 2173-2182
Bioline Code: pr16287
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 15, No. 10, 2016, pp. 2173-2182
© Copyright 2016 - Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria.
Neuroprotective effect of paeonol against isoflurane-induced neuroapoptosis and cognitive dysfunction|
Zhang, Jian-Xin; Li, Zhi-Ying; Zhao, Liang; Li, Gang; Cao, Gui-Lin & Zhang, Chuan-Yang
Purpose: To investigate whether paeonol affords neuroprotection against isoflurane-induced
Methods: Separate groups of neonatal rat pups were administered paeonol (20, 40 or 80 mg/kg) from
post-natal day 3 (P3) to post-natal day 15. On post-natal day 7, the pups were exposed to 6 h of
isoflurane (0.75 %) anesthesia. TUNEL assay was performed to assess neuroapoptosis. Cleaved
caspase-3 expressions were evaluated by immunohistochemistry and western blotting analysis. The
expressions of apoptotic pathway proteins and mitogen activated protein kinases (MAPKs) were
assessed by western blotting. The general behaviour of the rats was determined by open field test and
elevated maze test. Y-maze test and Morris water maze tests were performed to evaluate working
memory and cognition.
Results: Isoflurane exposure caused (p < 0.05) severe neuronal apoptosis in the hippocampal region
and enhanced caspase-3 expressions. Paeonol supplementation remarkably (p < 0.05) reduced
neuronal apoptosis and modulated expressions of apoptotic proteins. The raised expressions of NF-κB,
TNF-α, IL-6 and IL-1β and significantly (p < 0.05) enhanced JNK/p38 signalling cascades were inhibited
by paeonol. The expression levels of ERK were not significantly (p < 0.05) changed, but there was
significant improvement in the general behaviour and working memory of the rats.
Conclusion: Paeonol significantly improves cognitive impairments and offers neuroprotection against
isoflurane-induced apoptosis via modulating JNK/ERK/p38 MAPK and NF-κB signaling pathways.
Apoptosis; Isoflurane; Neurodegeneration; Paeonol; Cognitive impairment; Signaling pathways
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