Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 15, No. 10, 2016, pp. 2235-2239
Bioline Code: pr16294
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 15, No. 10, 2016, pp. 2235-2239
© Copyright 2016 - Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria.
Determination of cordycepin content of Cordyceps militaris recombinant rice by high performance liquid chromatography|
Wang, Xuesong; Liu, Fang; Li, Fang; Cai, Hongyan; Sun, Wei; Chen, Xuan; Gao, Hong & Shen, Wangyang
Purpose: To assess the suitability of high performance liquid chromatography (HPLC) for the
determination of cordycepin content of Cordyceps militaris recombinant rice
Methods: Cordyceps militaris recombinant rice was made by mixing brown rice with artificial Chinese
caterpillar fungus culture medium powder using twin-screw extrusion technology. Cordycepin content
was determined by reversed-phase HPLC with water:acetonitrile (95:5, v/v) as mobile phase, detection
wavelength of 260 nm, and flow rate of 1.0 mL/min.
Results: Cordycepin contents showed good linearity in the range of 1 - 50.0 μg/mL (r2 = 0.9996), and
while recovery ranged from 103.2 to 109.9 %. Relative standard deviation (RSD), precision and
repeatability RSD was 2.38, 0.76 and 1.46 %, respectively.
Conclusion: The HPLC method is simple, fast, accurate and reproducible. It is suitable for
determination of cordycepin content of artificial Chinese caterpillar fungus culture medium and brown
rice recombinant rice.
Recombinant rice; Cordycepin; Chinese caterpillar fungus; Aweto; Cordyceps militaris
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