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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 10, 2016, pp. 2235-2239
Bioline Code: pr16294
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 10, 2016, pp. 2235-2239

 en Determination of cordycepin content of Cordyceps militaris check for this species in other resources recombinant rice by high performance liquid chromatography
Wang, Xuesong; Liu, Fang; Li, Fang; Cai, Hongyan; Sun, Wei; Chen, Xuan; Gao, Hong & Shen, Wangyang

Abstract

Purpose: To assess the suitability of high performance liquid chromatography (HPLC) for the determination of cordycepin content of Cordyceps militaris recombinant rice
Methods: Cordyceps militaris recombinant rice was made by mixing brown rice with artificial Chinese caterpillar fungus culture medium powder using twin-screw extrusion technology. Cordycepin content was determined by reversed-phase HPLC with water:acetonitrile (95:5, v/v) as mobile phase, detection wavelength of 260 nm, and flow rate of 1.0 mL/min.
Results: Cordycepin contents showed good linearity in the range of 1 - 50.0 μg/mL (r2 = 0.9996), and while recovery ranged from 103.2 to 109.9 %. Relative standard deviation (RSD), precision and repeatability RSD was 2.38, 0.76 and 1.46 %, respectively.
Conclusion: The HPLC method is simple, fast, accurate and reproducible. It is suitable for determination of cordycepin content of artificial Chinese caterpillar fungus culture medium and brown rice recombinant rice.

Keywords
Recombinant rice; Cordycepin; Chinese caterpillar fungus; Aweto; Cordyceps militaris

 
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