Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 15, No. 11, 2016, pp. 2357-2363
Bioline Code: pr16311
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 15, No. 11, 2016, pp. 2357-2363
© Copyright 2016 - Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria.
Isolation and characterization of multidrug-resistant side population cells in prostate carcinoma|
Wang, Yu-qiu; Tian, He; Yang, Tong-ju & Liu, Li
Purpose: To isolate and characterize cancer stem-like side population (SP) cells from prostate cancer
tissues using Hoechst 33342 dye exclusion.
Methods: The presence of SP cells was analyzed in tumor samples by fluorescence activated cell
sorting. The cell survival rate and ability for cell self-renewal using the sphere formation assay were
evaluated after treatment with multiple drugs.
Results: SP cells in the prostate cancer samples constituted 2.8 %, but fell to 0.6 % after treatment with
verapamil. The SP cells showed high resistance to drugs such as 5-fluorouracil, cisplatin, paclitaxel (2
μmol/L) and oxaliplatin. The survival rate of SP cells after treatment with these drugs was significantly
higher (p < 0.01) than that of non-SP cells. Furthermore, the number of spheres generated in serum-free
medium was significantly higher in prostate cancer SP cells than in non-SP cells.
Conclusion: The presence of SP cells is responsible for prostate treatment failure and tumor
recurrence. Therefore, isolation and characterization of SP cells may provide new insights into the
development of novel therapeutic agents targeting cancer stem cells for complete eradication of the
Side population cells; ABC transporters; Cancer stem cells; Chemotherapy; Prostate treatment failure; Tumor recurrence; Drug resistance
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