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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 11, 2016, pp. 2371-2381
Bioline Code: pr16313
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 11, 2016, pp. 2371-2381

 en In vitro and in vivo hepatoprotective activity of extracts of aerial parts of Bidens pilosa check for this species in other resources L (Asteraceae)
Abdel-Ghany, Rasha Hassan; Barakat, Waleed Mohammed; Shahat, Abdelaaty Abdelaziz; Abd-Allah, Walid El-Sayed & Ali, Elzahraa Atef

Abstract

Purpose: To investigate the protective effects of aqueous and methanol extracts of Bidens pilosa using various in vivo and in vitro models of hepatic injury.
Methods: One kilogram of the aerial parts of Bidens pilosa was used to prepare 80 % methanol and aqueous extracts of the plant (500 g for each extract). The total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity of both extracts were evaluated. The hepatoprotective activity of these extracts in carbon tetrachloride (CCl4, 0.1 %) and D-galactosamine (700 mg/kg)-induced liver injury, respectively, was investigated in mice. Paracetamol-induced liver injury was used as in vitro reference standard.
Results: TPC and TFC of methanol extract were higher than those of the aqueous extract. The combination of methanol extract and silymarin showed the highest antioxidant activity. In vivo administration of CCl4 and D-galactosamine significantly increased the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP), but decreased the total protein, albumin and glutathione (GSH) contents of liver. Co-administration of the extracts (50 mg/kg) and silymarin (100 mg/kg) effectively countered the effects of CCl4 and D-galactosamine, while also exerting their antioxidant properties. Both methanol and aqueous extracts showed hepatoprotective activity in paracetamol-induced cytotoxicity in primary cultures of rat hepatocytes.
Conclusion: Bidens pilosa possesses significant in vivo and in vitro hepatoprotective activity in mice and may be therapeutically useful as a protective agent in acute liver injury.

Keywords
Bidens pilosa; D-galactosamine; Carbon tetrachloride; Paracetamol; Liver injury; Hepatoprotective; Antioxidant; Silymarin; Hepatocytes

 
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