| en |
Anti-thrombotic and anti-tumor effect of water extract of caulis of Sargentodoxa cuneata  (Oliv) Rehd et Wils (Lardizabalaceae) in animal models
Chen, Hong; Wan, Xue-mei & Zhou, Xue-lei
Abstract
Purpose: To investigate the anti-thrombosis and anti-tumor effect of the water extract of the caulis of
Sargentodoxa cuneata (Oliv.) Rehd. et Wils. (WCSW) in rat and mouse models.
Methods: WCSW extract was prepared and the main constituents were determined by high pressure
liquid chromatography (HPLC). The acute toxicity of the extract was determined in mice. Platelet
aggregation in rat platelet-rich plasma (PRP) was examined to evaluate the effect of the extract on
platelet function. Thereafter, the cytotoxic activity of WCSW on HL60, A549, S180 and H22 cells was
determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In vivo anti-tumor
effect of WCSW was further evaluated on H22 cells transplanted in mice, while the expression of
caspase-3, caspase-9, Bcl-2 and Bax proteins were assayed by Western blot analysis.
Results: Protocatechuic acid, rhodiola glucoside and chlorogenic acid were identified as the main
constituents of WCSW. Platelet aggregation was significantly inhibited by treatment with the extract at
concentrations of 1, 5 and 10 mg/mL. WCSW also showed significant inhibitory effect on HL60, A549,
S180 and H22 cells in vitro with half maximal inhibitory concentration (IC50 value of 321.9, 285.0, 130.3
and 76.1 μg/mL, respectively. Furthermore, WCSW exhibited obvious anti-tumor effect on H22
transplanted tumor in vivo. After treatment with WCSW, caspase-3, caspase-9 and Bax were
significantly (p < 0.05) up-regulated, whereas Bcl-2 was significantly (p < 0.05) down-regulated in the
tumor tissues.
Conclusion: WCSW possesses significant antithrombosis and anti-tumor effect, and therefore, has the
potentials to be developed into effective drugs for clinical treatment of cancer and thrombosis diseases.
Keywords
Sargentodoxa cuneata; Anti-thrombosis; Anti-tumor; Platelet aggregation; Apoptosis; Caspase; Protocatechuic acid; Rhodiola glucoside; Chlorogenic acid
|
