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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 11, 2016, pp. 2445-2449
Bioline Code: pr16322
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 11, 2016, pp. 2445-2449

 en Effect of Turkish pollen and propolis extracts on caspase-3 activity in myeloid cancer cell lines
Uçar, Meltem; Değer, Orhan; Gerigelmez, Asuman Yiğit; Cengiz, Sevil; Barlak, Yaşam & Ovalı, Ercüment

Abstract

Purpose: To investigate the apoptosis-inducing capacity of dimethyl sulfoxide (DMSO) extracts of bee pollen and propolis in HL-60 Myeloid Cancer Cell Lines.
Methods: DMSO extracts of pollen and propolis were incubated separately with HL-60 cells, and caspase-3 activity evaluated. In order to determine the cell cycle characteristics of HL-60 cells with and without extracts of pollen and propolis, the cells were analysed using flow cytometry.
Results: The DMSO extract of propolis (0.5 mg/mL) increased apoptosis from undetectable levels to 60.1 %, while maintaining cell viability. The DMSO extract of pollen (2 mg/ml) increased apoptosis from undetectable levels to 52.2 % while decreasing cell viability by 62 %. Caspase-3 activity in HL-60 cells incubated with DMSO extracts of pollen and propolis were 3.6- to 12-fold higher than in controls.
Conclusion: Turkish pollen and propolis individually increase apoptosis and the activity of caspase-3 in HL-60 cells. This finding indicates that bee products may have beneficial effects in the treatment of cancer.

Keywords
Pollen; Propolis; Apoptosis; Caspase-3; Myeloid Cancer

 
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