Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 15, No. 11, 2016, pp. 2475-2481
Bioline Code: pr16326
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 15, No. 11, 2016, pp. 2475-2481
© Copyright 2016 - Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria.
Simultaneous determination of isoniazid and pyrazinamide in plasma by high performance liquid chromatography|
Mahjoub, Abdallah A; Khan, Amer H; Sulaiman, Syed A Syed; Lajis, Razak; Man, Che N & Ali, Irfhan A Hyder
Purpose: To develop and validate a new high performance liquid chromatographic (HPLC) method for
the simultaneous determination of isoniazid (INH) and pyrazinamide (PZA) in plasma.
Methods: A 150 μL aliquot of plasma was mixed with 75 μL of 10 % trichloroacetic acid containing 100
mg/L of acetanilide as the internal standard (IS). After vortex mixing and centrifugation, 100 μL of the
supernatant was reacted with 20 μL of 0.1 % trans-cinnamaldehyde for 10 min, and then 40 μL of 1M
ammonium acetate was added. Finally, 20 μL was injected into the HPLC system. HPLC analysis was
performed on reversed phase C18 column. The initial composition of the mobile phase was 4 %
acetonitrile, and 96 % of 20 mM 1-hexane sulfonic acid (PH 2.7) delivered at a flow rate 1 mL/min.
Results: All calibration curves were linear (r2 > 0.997). The method was accurate, and relative error
(RE) was < 4.5 % for both drugs. Intra-day and inter-day precision was good for both drugs, with the
highest relative standard deviation (RSD) being 8.51 %. The lower limit of quantification was 0.60 mg/L
for isoniazid and 3.00 mg/L for pyrazinamide.
Conclusion: The method proposed here is precise, accurate, fast, simple and suitable for therapeutic
drug monitoring of INH and PZA simultaneously.
HPLC; Isoniazid; Pyrazinamide; Plasma; Simultaneous analysis
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