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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 12, 2016, pp. 2535-2542
Bioline Code: pr16333
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 12, 2016, pp. 2535-2542

 en Expression profiling of miR-96, miR-584 and miR-422a in colon cancer and their potential involvement in colon cancer pathogenesis
Lu, Min; Zhou, Xuan; Zheng, Cheng-Guo & Liu, Feng-Jun

Abstract

Purpose: To determine the correlation between miRNAs; miR-96, miR-422a and miR584, and colon cancer, and also to test whether any of these miRNAs can act as non-invasive biomarkers in colon cancer
Methods: The tumor samples and the corresponding normal mucosa used in this study were collected from 60 patients diagnosed with colon cancer. HT-29, HCT-116 and SW-620 cell lines were used for miRNA expression profiling. Total RNA was extracted using kit-based methods with minor modifications, and specific miRNAs were detected and quantified by quantitative real-time polymerase chain reaction [RT-PCR].
Results: The results indicate down-regulation of miR-96, miR-584 and miR-422a in colon cancer tissue and decreased expression levels in the three colon cancer cell lines (all p < 0.01). Lower miRNA expression levels are correlated with increased tumor size.
Conclusion: This study shows that miR-96, miR-584 and miR-422a are down-regulated in colon cancer and are associated with tumor size. Thus, the ratio of miR-96/miR-638 in plasma is a potential non-invasive biomarker in colon cancer.

Keywords
Colon cancer; miR-96; miR-422a; miR-584; Expression profiling; Biomarker

 
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