Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 15, No. 12, 2016, pp. 2647-2652
Bioline Code: pr16348
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 15, No. 12, 2016, pp. 2647-2652
© Copyright 2016 - Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria.
Modulatory effect of Althaea officinalis L root extract on cisplatin-induced cytotoxicity and cell proliferation in A549 human lung cancer cell line|
Zhang, Yukun; Kong, Fanhua; Zhang, Lei; Li, Chao & Zhang, Runqi
Purpose: To explore the modulatory effect of an Althaea officinalis root extract (AORE) on cisplatininduced
cytotoxicity and cell proliferation in a lung cancer cell line.
Methods: Aqueous AORE was obtained from peeled and powdered roots. The effect of cisplatin on
cytotoxicity and cell proliferation was studied. The cisplatin concentrations tested ranged from 0 - 30
mg/mL. Cell viability and proliferation were studied using trypan blue and MTT assays, respectively. The
cells were also exposed to a combination of cisplatin and the AORE.
Results: Cisplatin yielded a 50 % inhibitory concentration at 25 mg/mL and exhibited a dose-dependent
cell proliferation loss. Combined use of the root extract and cisplatin had significant (R2 = 0.8305)
modulatory effects on cytotoxicity and antiproliferative activities. The best inhibitory effects were
observed in cells exposed to a combination of 8 or 10 % AORE and 25 mg/mL cisplatin. The optimal
effect on cell proliferation was obtained using 25 mg/mL cisplatin and 10 % v/v AORE.
Conclusion: The enhanced activity of cisplatin in combination with AORE was more pronounced for
cell proliferation than cytotoxicity, indicating that AOREs may be used to control tumor progression and
A549 cells; Althaea officinalis; lung cancer; cell proliferation; cell viability; cisplatin; modulatory effect
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