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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 12, 2016, pp. 2647-2652
Bioline Code: pr16348
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 12, 2016, pp. 2647-2652

 en Modulatory effect of Althaea officinalis check for this species in other resources L root extract on cisplatin-induced cytotoxicity and cell proliferation in A549 human lung cancer cell line
Zhang, Yukun; Kong, Fanhua; Zhang, Lei; Li, Chao & Zhang, Runqi

Abstract

Purpose: To explore the modulatory effect of an Althaea officinalis root extract (AORE) on cisplatininduced cytotoxicity and cell proliferation in a lung cancer cell line.
Methods: Aqueous AORE was obtained from peeled and powdered roots. The effect of cisplatin on cytotoxicity and cell proliferation was studied. The cisplatin concentrations tested ranged from 0 - 30 mg/mL. Cell viability and proliferation were studied using trypan blue and MTT assays, respectively. The cells were also exposed to a combination of cisplatin and the AORE.
Results: Cisplatin yielded a 50 % inhibitory concentration at 25 mg/mL and exhibited a dose-dependent cell proliferation loss. Combined use of the root extract and cisplatin had significant (R2 = 0.8305) modulatory effects on cytotoxicity and antiproliferative activities. The best inhibitory effects were observed in cells exposed to a combination of 8 or 10 % AORE and 25 mg/mL cisplatin. The optimal effect on cell proliferation was obtained using 25 mg/mL cisplatin and 10 % v/v AORE.
Conclusion: The enhanced activity of cisplatin in combination with AORE was more pronounced for cell proliferation than cytotoxicity, indicating that AOREs may be used to control tumor progression and metastasis.

Keywords
A549 cells; Althaea officinalis; lung cancer; cell proliferation; cell viability; cisplatin; modulatory effect

 
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