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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 15, No. 12, 2016, pp. 2659-2667
Bioline Code: pr16350
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 15, No. 12, 2016, pp. 2659-2667

 en Diversity of archaea and bacteria in a biogas reactor fed with Pennisetum sinese Roxb by 16S rRNA sequence analysis
Zhao, Chao; Ai, Chao; Li, Qiuzhe; Yang, Chengfeng; Zhou, Guangzhi & Liu, Bin

Abstract

Purpose: To investigate the structure and function of the complex rumen microbial community in a biogas reactor by 16S rRNA gene analysis, which was fed with Pennisetum sinese Roxb as the monosubstrate.
Methods: Two 16S ribosomal RNA (rRNA) clone libraries of bacteria and archaea were established by polymerase chain reaction. Community structure was determined by phylogenetic analyses of 119 and 100 16S rRNA gene clones from the bacterial and archaeal libraries, respectively.
Results: In the bacterial library, 13.4 % of clones were affiliated with Treponema porcinum check for this species in other resources , 5.9 % with Eubacterium limosum check for this species in other resources , 5 % with Clostridium, 5 % with Bacteroidetes, 4.2 % with Firmicutes, 2.5 % with Anaerofilum and a total of 64 % clones belonged to unclassified or uncultured bacteria. In the archaeal library, Methanobacterium curvum made up 12 % of known clones, Methanosarcina barkeri check for this species in other resources represented 8 %, Methanobacterium bryantii check for this species in other resources represented 4 % and Methanofollis ethanolicus check for this species in other resources represented 2 %, respectively; the remaining 74 % of the clones were unclassified archaebacteria.
Conclusion: T. porcinum and M. curvum are the most predominant bacteria and archaea in a biogas reactor fed with P. sinese as the sole substrate.

Keywords
Pennisetum sinese Roxb; Archaea, Bacteria; Biogas reactor; 16S rDNA sequencing

 
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