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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 16, No. 1, 2017, pp. 59-65
Bioline Code: pr17008
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 16, No. 1, 2017, pp. 59-65

 en Isolation of nematicidal constituents from essential oil of Kaempferia galanga check for this species in other resources L rhizome and their activity against Heterodera avenae Wollenweber check for this species in other resources
Li, Yun Chao; Ji, Hua; Li, Xiu Hua; Zhang, Hai Xin & Li, Hong Tao

Abstract

Purpose: To explore the nematicidal activities of the essential oil of Kaempferia galanga rhizomes and its isolated constituents against Heterodera avenae
Methods: Essential oil of K. galanga rhizomes was obtained by hydrodistillation and characterized by gas chromatography/mass spectrometric (GC/MS) analysis using HP-5MS column. Evaluation of nematicidal toxicity was performed against juveniles (J2) of H. avenae. The bioactive constituent compounds were isolated and identified from the oil based on bioactivity-directed fractionation.
Results: Forty-one components were identified and the main components of the essential oil of K. galanga are as follows: ethyl-ρ-methoxy cinnamate (34.79 %), ethyl cinnamate (20.72%), 1,8-cineole (8.96 %), trans-cinnamaldehyde (7.03%) and borneol (5.64 %). The essential oil exhibited nematicidal activity against the cereal cyst nematode with an LC50 value of 91.78 μg/mL. Ethyl cinnamate, ethyl ρ-methoxy cinnamate and trans-cinnamaldehyde (median lethal concentration LC50 = 100.60 μg/ml, 83.04 μg/mL and 94.75 μg/mL, respectively) exhibited stronger nematicidal toxicity than borneol (LC50 = 734.89 μg/mL) and 1,8-cineole (LC50 = 921.21 μg/mL) against the cereal cyst nematode.
Conclusion: The results indicate that the essential oil of K. galanga and its isolated constituents have a potential for development into natural nematicides for the control of cereal cyst nematodes.

Keywords
Kaempferia galanga; Heterodera avenae; Nematicidal activity; Cereal cyst nematodes; Ethyl cinnamate; Ethyl ρ-methoxy cinnamate; Trans-cinnamaldehyde

 
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