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Extraction and characterization of radish seed oils using different methods Zhao, Gongling; Ren, Yupeng & Ma, Hanjun Abstract Purpose: To evaluate the impact of three different extraction methods on oil yield, physicochemical properties and bioactive ingredients of radish seeds. Methods: Radish seed oil was prepared by traditional solvent extraction (SE), supercritical carbon dioxide extraction (SCE) and sub-critical propane extraction (SPE). The yield, physicochemical properties, fatty acid composition and oxidative stability of the oil extracts were compared. The contents of tocopherol and sulforaphene in the oils were also determined. Results: The oil yield obtained by SPE, SE, SCE were 33.69, 27.17 and 24.10 %, respectively. There were no significant differences in physicochemical properties and fatty acid composition of oils extracted by the three methods. However, SCE oil had the best oxidative stability, and highest contents of vitamin E and sulforaphene, followed by oils from SPE and SE Conclusion: SCE is highly selective for tocopherol and sulforaphene, which could explain its high oil oxidative stability. These results suggest that of the three extraction methods, SCE is best suited for preparing medicinal radish seed oil. Keywords Radish seed oil; Different extraction methods; Fatty acid composition; Tocopherol; Sulforaphene

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