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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 16, No. 2, 2017, pp. 255-261
Bioline Code: pr17034
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 16, No. 2, 2017, pp. 255-261

 en Antibodies against Shigella flexneri check for this species in other resources adhesion molecule outer membrane protein (OMP) can cross-react with OMPs of some Shigella species
Milliana, Alvi; Noorhamdani, AS; Poeranto, Sri; Handono, Kusworini; Prawiro, Sumarno Reto; Fitrianingsih, Avin Ainur & Rachma, Lailia Nur

Abstract

Purpose: To identify a Shigella flexneri hemagglutinin outer membrane protein (OMP) as an adhesion factor and examine its ability to cross-react with the OMPs of other Shigella species.
Methods: OMP was isolated from the bacterium S. flexneri after shaving the pili using a pili bacterial cutter in a solution of 0.5 % n-octyl-β-D-glucopyranoside. Hemagglutination was evaluated using mice erythrocytes. The molecular weight (MW) of the OMPs was profiled by SDS-PAGE. Adhesion molecules were identified by calculating the index of adhesion. Thereafter, antigen cross-reaction with antibodies was examined by Western blotting,
Results: The S. flexneri adhesion molecule OMP has a MW of 28 kDa. An antibody against the 28 kDa S. flexneri OMP adhesion molecule cross-reacted with different MW OMPs from other Shigella species.
Conclusion: This study showed that the 28 kDa S. flexneri OMP is a hemagglutinin adhesion protein, and antibodies against it can cross-react with the OMPs of other Shigella species.

Keywords
Shigella flexneri OMP; Outer membrane protein; Protein adhesion; Antibody; Cross-reaction

 
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