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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996 EISSN: 1596-5996
Vol. 16, No. 2, 2017, pp. 255-261
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Bioline Code: pr17034
Full paper language: English
Document type: Research Article
Document available free of charge
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Tropical Journal of Pharmaceutical Research, Vol. 16, No. 2, 2017, pp. 255-261
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Antibodies against Shigella flexneri adhesion molecule outer membrane protein (OMP) can cross-react with OMPs of some Shigella species
Milliana, Alvi; Noorhamdani, AS; Poeranto, Sri; Handono, Kusworini; Prawiro, Sumarno Reto; Fitrianingsih, Avin Ainur & Rachma, Lailia Nur
Abstract
Purpose: To identify a Shigella flexneri hemagglutinin outer membrane protein (OMP) as an adhesion
factor and examine its ability to cross-react with the OMPs of other Shigella species.
Methods: OMP was isolated from the bacterium S. flexneri after shaving the pili using a pili bacterial
cutter in a solution of 0.5 % n-octyl-β-D-glucopyranoside. Hemagglutination was evaluated using mice
erythrocytes. The molecular weight (MW) of the OMPs was profiled by SDS-PAGE. Adhesion molecules
were identified by calculating the index of adhesion. Thereafter, antigen cross-reaction with antibodies
was examined by Western blotting,
Results: The S. flexneri adhesion molecule OMP has a MW of 28 kDa. An antibody against the 28 kDa
S. flexneri OMP adhesion molecule cross-reacted with different MW OMPs from other Shigella species.
Conclusion: This study showed that the 28 kDa S. flexneri OMP is a hemagglutinin adhesion protein,
and antibodies against it can cross-react with the OMPs of other Shigella species.
Keywords
Shigella flexneri OMP; Outer membrane protein; Protein adhesion; Antibody; Cross-reaction
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