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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 16, No. 2, 2017, pp. 263-269
Bioline Code: pr17035
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 16, No. 2, 2017, pp. 263-269

 en Biosynthesis of lovastatin using agro-industrial wastes as carrier substrates
Javed, Sadia; Meraj, Munazzah; Mahmood, Saqib; Hameed, Arruje; Naz, Farah; Hassan, Sameera & Irfan, Rao

Abstract

Purpose: To compare fungal strains including Aspergillus flavipes check for this species in other resources GCBL-72, Aspergillus flavus check for this species in other resources GCBL-60, and Aspergillus niger check for this species in other resources GCBL-45 and determine whether solid- or liquid-state fermentation (SSF or LSF) is more appropriate for lovastatin production using various inexpensive raw materials.
Methods: LSF and SSF techniques were used to produce the drug lovastatin. High-performance liquid chromatography was performed out to quantify lovastatin production. A kinetic growth model was applied to estimate product formation at the expense of substrate utilization.
Results: Aspergillus flavus GCBL-60 was a superior lovastatin-producing strain consuming wheat bran as the raw material in SSF. The optimum lovastatin production was 28.36 ± 0.76 mg/100mL at 35 °C, pH 5.5, inoculum size 2 mL, 96 h incubation time, and 60 % moisture content. Evaluation of the kinetic growth parameters for lovastatin production confirmed that product formation was improved after fermentation parameter optimization.
Conclusion: Our results indicate that Aspergillus flavus GCBL-60 was best lovastatin-producing strain and that SSF was superior to LSF for maximum production. Careful optimization can enhance product formation.

Keywords
Hypercholesterolemia; Kinetics; Optimization; Lovastatin; Solid-State Fermentation; Raw materials

 
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