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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996 EISSN: 1596-5996
Vol. 16, No. 2, 2017, pp. 279-286
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Bioline Code: pr17037
Full paper language: English
Document type: Research Article
Document available free of charge
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Tropical Journal of Pharmaceutical Research, Vol. 16, No. 2, 2017, pp. 279-286
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Baicalein and U0126 suppress bladder cancer proliferation via MAPK signaling pathway
Tang, Xiaoping; Chen, Jiexiang; Yu, Xiaolan; Tang, Li; Li, Yan & Xia, Jiyi
Abstract
Purpose: To investigate baicalein and 1,4-diamino-2,3-dicyano-1,4-bis[2-aminophenylthio] butadiene
(U0126)effects on human bladder cell line T24 proliferation and related mechanisms.
Methods: Twenty micromoles of baicalein or 10 μM U0126 were incubated with T24 cells. Cell viability
was tested by CCK8 assay. Cell cycle was evaluated by flow cytometry while cell apoptosis was
detected by Annexin V/PI and TUNEL assay. MAPK signaling pathway was evaluated by real time
polymerase chain reaction (RT-PCR) and western blot.
Results: Baicalein and U0126 suppressed bladder cancer cell T24 proliferation by blocking cell cycle in
G0~G1 phase. TUNEL and Annexin V/PI detection showed both baicalein and U0126 induced T24 cell
apoptosis. Baicalein and U0126 significantly down-regulated MAPK signaling pathway related molecule
activity in both mRNA and protein levels (p < 0.05).
Conclusion: Baicalein and U0126 restrain bladder cancer cell proliferation and promote cell apoptosis
by affecting MAPK signaling pathway. Thus, they have potentials for use in the treatment of bladder
cancer.
Keywords
Bladder cancer; Baicalein; 1,4-diamino-2,3-dicyano-1,4-bis[2-aminophenylthio] butadiene; MAPK signal pathway; Apoptosis
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