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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 16, No. 2, 2017, pp. 337-342
Bioline Code: pr17044
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 16, No. 2, 2017, pp. 337-342

 en Luteolin as a potent anti-leishmanial agent against intracellular Leishmania tropica parasite
Iqbal, Kashif; Jamal, Qaiser; Iqbal, Javeid; Afreen, Maria Sadaf; Sandhu, Muhammad Zeeshan Ahmed; Dar, Eshwa; Farooq, Umar; Mushtaq, Mohammad Fahd; Arshad, Numera & Iqbal, Muhammad Mohsin


Purpose: To examine the anti-leishmanial and cytotoxic effects of five naturally occurring phenolic compounds: luteolin (1), lalioside (2), luteolin-4’-O-β-D-glucopyranoside (3), apigenin 4-O-β-D-glucopyranoside (4) and apigenin (5) on Leishmania tropica KWH23 amastigotes .
Methods: The compounds were isolated from the leaves of Lawsonia Inermis check for this species in other resources via hyphenated high performance liquid chromatography-high resolution mass spectrometry coupled with solid phase extraction-tube transfer nuclear magnetic resonance technique. The isolated compounds were given intraperitoneally to L. tropica KWH23 amastigotes-infected albino mice at a dose of ≥ 3 mg/kg for 5 days. Amphotericin-B was used as standard (reference) drug. Lymphocytes were used to analyze their cytotoxicity.
Results: For compound 1, mean lesion size decreased from 0.82 ± 0.12 to 0.10 ± 0.01 after 120 days, with 97 % cure of intracellular L. tropica amastigotes at a dose of 15 mg/kg, compared to amphotericin B which produced 95 % cure at a dose of 30 mg/kg. Half-maximal concentration (IC50) for compound 1 was 4.15 μg/ml against lymphocytes.
Conclusion: The results indicate that luteolin is a potent inhibitor of L. tropica amastigotes, with a higher cytotoxic activity against lymphocytes, compared with luteolin-4’-O-β-D-glucopyranoside.

Leishmania tropica; Luteolin; Lalioside; Luteolin-4’-O-β-D-glucopyranoside; Apigenin 4-O- β-D-glucopyranoside; Apigenin

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