Hepatoprotective effect of Fufang-Huanglu oral liquid on α-naphthylisothiocyanate-induced hepatitis jaundice in mice

Purpose: To investigate the effect of Fufang-Huanglu Oral Liquid (HOL) on hepatitis jaundice in mice.
Methods: A total of 72 mice were divided into 6 groups (n = 12): normal group, control group (model group), positive-treated group, and 3 HOL treatment groups (7.5, 15 and 30 mL/kg). Mice in normal and control groups received normal saline (20 mL/kg) orally, while positive and HOL-treated mice were orally administered Huganning tablets (1.0 g/kg) and HOL (7.5, 15 and 30 mL/kg), respectively. After 8 days, all mice (except normal group) were orally administered α-naphthylisothiocyanate (ANIT, 100 mg/kg) to induce hepatitis jaundice, and sacrificed 2 days after drug administration. Serum GPT, GOT and TNF-α, as well as liver index, MDA, SOD and lipid profiles were determined.
Results: The results showed that HOL, at all doses, significantly decreased liver index, serum GPT, serum SGOT and serum TNF-α (p < 0.01). HOL also significantly decreased MDA, total cholesterol, TC and triglycerides, TG (p < 0.01), but increased liver SOD (p < 0.01). Histological results indicate that HOL ameliorated liver injury induced by ANIT.
Conclusion: These results showed that HOL possesses significant hepatoprotective effects against liver injury.

Keywords
Fufang-Huanglu Oral Liquid; Hepatoprotective; Mice; Hepatitis; Jaundice; α-Naphthylisothiocyanate; Liver index