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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996 EISSN: 1596-5996
Vol. 16, No. 2, 2017, pp. 349-356
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Bioline Code: pr17046
Full paper language: English
Document type: Research Article
Document available free of charge
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Tropical Journal of Pharmaceutical Research, Vol. 16, No. 2, 2017, pp. 349-356
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Genistein attenuates ischemia/reperfusion injury in rat kidneys via enhancement of antioxidant defense mechanisms: Activation of Nrf-2/HO-1 signaling
Shou-Liang, Wang; Duan, Lian; Li, Wei; Wang, Gong-Ming & Zhang, Meng-Yuan
Abstract
Purpose: To investigate the protective role of genistein against ischemic reperfusion (I/R) injury in rat
kidneys.
Methods: Group I (control, n = 10) consisted of animals that were not operated on while group II (sham,
n = 10) were animals surgically operated on, similar to I/R group without renal bilateral ischemia. Group
III (genistein, n = 10) consisted of animals administered 10 mg/kg genistein by oral gavage for 7
consecutive days while group IV (I/R, n = 10) animals were subjected to 45 min of renal bilateral
ischemia followed by 24 h of reperfusion. Group V (genistein+I/R, n = 10) received 10 mg/kg genistein
by oral gavage for 7 consecutive days and then subjected to 45 min of renal bilateral ischemia followed
by 24 h of reperfusion. Renal function, total oxidant capacity and total antioxidant status in serum were
evaluated in the rats. Further, reactive oxygen species generation as well as levels of protein carbonyl,
lipid peroxidation, and enzymatic and non-enzymatic antioxidants were determined. Nrf-2 (nuclear factor
(erythroid-derived 2)-like 2) and HO-1 (Heme oxygenase-1) expressions were determined by western
blot.
Results: Pre-treatment with genistein (10 mg/kg) significantly (p < 0.001) ameliorated I/R induced renal
damage by reducing the levels of serum markers. Genistein pre-treatment significantly decreased (p <
0.001) I/R injury induced-ROS, lipid peroxides and protein carbonyl content (p < 0.001). I/R injury
significantly (p < 0.001) decreased non-enzymatic and enzymatic antioxidant activities. Genistein pre-treatment
also prevented renal I/R injury by significantly up-regulating Nrf-2, HO-1 expressions and
antioxidant status.
Conclusion: Thus, genistein may be therapeutically useful against kidney I/R injury by improving
antioxidant defense mechanisms.
Keywords
Oxidative stress; Genistein; Ischemic reperfusion injury; Renal damage; Antioxidant; Nuclear factor (erythroid-derived 2)-like 2; Heme oxygenase-1; Nrf-2; HO-1
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