search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 16, No. 2, 2017, pp. 387-396
Bioline Code: pr17051
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 16, No. 2, 2017, pp. 387-396

 en Community of endophytic fungi from the medicinal and edible plant Fagopyrum tataricum check for this species in other resources and their antimicrobial activity
Zhong, Ling Yun; Zou, Liang; Tang, Xiao Hui; Li, Wen Fei; Li, Xing; Zhao, Gang & Zhao, Jiang Lin

Abstract


Purpose: To isolate and identify the fungal endophytes of Fagopyrum tataricum and assess their antimicrobial activity.
Methods: The fungal isolates were identified according to their morphological characters and internal transcribed spacer (ITS) sequence analysis. Both broth-dilution-colorimetric assay and spore germination test were employed to assess the antimicrobial activity of fungal mycelia extracts..
Results: A total of 72 endophytic fungal isolates were successfully obtained from the healthy tissues of F. tataricum. On the basis of their morphological and molecular characteristics, thirteen distinct fungal isolates were identified, and belonged to Alternaria check for this species in other resources , Bionectria check for this species in other resources , Botryosphaeria check for this species in other resources , Fusarium check for this species in other resources , Guignardia check for this species in other resources , Nectria check for this species in other resources , Neonectria check for this species in other resources , Phomopsis check for this species in other resources , Pseudocercospora check for this species in other resources and Verticillium check for this species in other resources spp. Of these, Pseudocercospora owned the maximum colonization frequency (15.3 %), next for Alternaria (12.5 %) and Neonectria (11.1 %). Through antimicrobial screening assays, six endophytes (Fataf-4, 6, 9, 11, 12 and 15) were active against Bacillus subtilis check for this species in other resources , Staphylococcus aureus check for this species in other resources , Agrobacterium tumefaciens check for this species in other resources , Escherichia coli check for this species in other resources and Pseudomonas lachrymans, and their minimal inhibitory concentration (MIC) ranged from 0.125 to 2.00 mg/mL. Furthermore, the endophytes, Fataf-9 and Fataf-11, also displayed strong inhibitory activity on the spore germination of F. oxysporum f. sp. vasinfectum check for this species in other resources and F. oxysporum f. sp. cucumerinum check for this species in other resources , and their median effective inhibitory concentration (IC50) was 0.356, 0.463, 0.451 and 0.489 mg/mL, respectively.
Conclusion: The endophytic fungi of F. tataricum appear to be diverse and promising in their antimicrobial activity, and may represent a potential source of antibiotics for agriculture and/or pharmaceutical applications.

Keywords
Endophytic fungi; Fagopyrum tataricum; Diversity; Antimicrobial activity; Antibiotics; Pseudocercospora

 
© Copyright 2017 - Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria.
Alternative site location: http://www.tjpr.org

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2022, Site last up-dated on 19-Jan-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil