To examine the effect of crude Polycephalomyces nipponicus
Cod-MK1201 mycelial extract
on the viability and cell surface morphology of methicillin-susceptible Staphylococcus aureus
and methicillin-resistant Staphylococcus aureus
Time-kill assays were conducted by incubating test bacteria with the extract and sampling at
selected time points within a 24 h period. The effects of the extract on MSSA and MRSA ultrastructure
were determined using a scanning electron microscope (SEM).
Time-kill assay data indicate a bactericidal effect against both strains of staphylococci. The
extracts were rapidly bactericidal at concentrations of 1 x MIC and 2 x MIC, achieving complete
elimination of the test bacterial strains within 2 h. SEM micrographs of S. aureus
taken after treatment
with various concentrations of the extract revealed extensive morphological alterations to the cell
surface of both MSSA and MRSA.
The results confirm the antibacterial activity of P. nipponicus
extract. Further research may allow this to be developed as an alternative therapy to alleviate S. aureus